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Modern
Techniques in Plant Genetic Engineering
Collaborations
in Transgenics
Does
your lab have an interest in gene functional analysis through transgenics?
Is your
lab interested in analyzing a cloned gene sequence through transgenics
for any of the following applications:
- Promoter
Analysis
- Trait
Modifications
- Trait
Improvement
- Mutational
Analysis
- Genomics
Applications
PLANTS
WE CAN TRANSFORM
- Rice
- Turf (Creeping
bentgrass, Colonial bentgrass, Tall Fescue, Velvet bentgrass, and Poa
annua)
- Soybean
- Corn
- Arabidopsis
HOW
DO WE ACCOMPLISH THIS?
- An Undergraduate
Laboratory Course
- Formal
Research Collaborations
COURSE
BACKGROUND
Modern
Techniques in Plant Biotechnology is a comprehensive two semester
course which provides students with a project-based approach to plant
biotechnology. In this course each student receives their own plant gene
expression vector to stably introduce into plants. The goal for each student
is to produce one hundred and fifty independent transgenic events and
conduct molecular and phenotypic characterizations. The projects are not
intended to be merely for training only but to yield meaningful scientific
results for publications. The course has been designed in two sections,
a laboratory component in which students learn the applied techniques
for genetic engineering as well as methods of analysis of plant gene expression
and a lecture component presenting current topics and approaches in plant
biotechnology. Historical background into the principals of genetic engineering
are presented during the lectures and many aspects of applied research
areas, patent development, commercialization of a product and agricultural
and environmental concerns are addressed. In the laboratory, state-of-the-art
techniques for plant biotechnology are presented via intensive hands-on
research.
Each student
is given a unique gene construct to be introduced into a turf grass species,
soybean, corn, or rice. The students conduct all the necessary cell culture
and transgenic procedures in order to recover stably transformed callus
by the end of the first semester. The second semester then involves the
regeneration of plants from the transformed callus as well as the molecular
analysis of tissue from those plants. With each successive year it is
anticipated that the scope and breadth of the projects will change significantly
based on new developments in the agricultural community.
SCOPE
OF RESEARCH CONDUCTED
Laboratory based experiments include such techniques as Agrobacterium
and biolistic-mediated transformation, tissue culture and DNA analysis
of the transformants including PCR, Southern, RT-PCR and Western analysis.
Each independent project is designed to present the course material while
students are involved in research that is currently pertinent to the agricultural
community. The scope of the projects is such that they should be able
to be completed within the two semester time frame. Each project consists
of two separate parts: the first being the introduction of the different
constructs into embryogenic callus including the regeneration of transgenic
plants; the second part consists on the molecular analysis of the transformants,
including both phenotypic analysis and tissue specific gene expression.
OUR
COLLABORATORS TO DATE:
- Dr. Steven
Dellaporta
Yale University, New Haven, CT
- Dr. Ajay
Garg
Cornell University, Ithaca, NY
- Dr. Ray
Wu
Cornell University, Ithaca, NY
- Dr. Michael
Neff
Washington University, St. Louis, MO
- Dr. Joanne
Chory
Salk Institute, San Diego, CA
- Dr. Hong
Luo
HybriGene Inc, Kingston, RI
- Dr. Joseph
Wipff
Pure Seed Testing Inc, Hubbard, OR
View us as one of your collaborators!
OUR COURSE CAN TRANSFORM THE PLANTS YOU NEED
Reliable transformation systems to produce transgenics are available for
rice, corn, soybean and several varieties of turf grass. If you have a plant
gene expression vector but lack the ability to transform the plant you need…
This is an opportunity for you to advance your research projects without
the need to develop the transformation expertise, saving you valuable time
and money.
WOULD YOU LIKE TO COLLABORATE WITH US?
We are interested in collaborative projects that can be utilized in our
course. We will provide the transgenic expertise and perform molecular
analysis. Do you have plant gene expression vectors but lack the facilities
to transform and analyze the plants.
We can help.
FOR
FURTHER INFORMATION CONTACT:
Dr. Albert Kausch
Phone: (401) 874.7121
Email: akausch@lifeedu.org
Dr. Joel
Chandlee
Phone: (401) 874.2529
Email: jch9826u@postoffice.uri.edu
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Modern
Techniques in Plant Genetic Engineering Brochure (pdf)
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