Modern Techniques in Plant Biotechnology: A model new laboratory course in biotechnology accomplished through project-based learning

Longo, Chip (A) (E) Bockstael, Andrew (D) Brady-Wood, Matthew (D) Delmonico, Courtney (B) DeNoia, Michael G (C) Deragon, Derek N (B) Eitas, Tim (B) Johnson, Jeffrey C (B) Kunajukr, Milan (C) Lickwar, Colin (B) LaMantia, Jonathan (B) Martin, Delia M (B) Martin, Jon (B) Peabody, Hillary (B) Pedraza, Jessica (C) Pizza, Christopher (B) Stevens, Gregg R. (D) Peterson, Courtney (D) Kausch, Albert P (A) (E)
Affiliations: (A): HybriGene Inc. (B): University of Rhode Island (C): New London High School (D): South Kingstown High School (E): life edu.org

We have created and piloted a new comprehensive experiential two-semester course which presents current topics and approaches in plant biotechnology (as a lecture component) and a project-based techniques approach to genetic engineering and analysis of plant gene expression (as a laboratory skills component). In the first semester, working in teams, students receive unique gene constructs to introduce into rice or grasses which address meaningful research problems (see accompanying abstracts). The students then conduct all the necessary cell culture and transgenic procedures to recover stably transformed cells by the end of the first semester and return for the second semester to conduct the molecular characterization and plant regeneration. The projects include the introduction of constructs for a functional genomics effort on rice and introduction of trait improvement genes into perennial rye grass and tall fescue. The laboratory experience provides students with all of the necessary techniques currently used in the growing industry of agricultural biotechnology as well as approaches for basic research using transgenics. Students have compiled a handbook of Standard Operating Procedures for the techniques and procedures in the course. This project-based approach and the array of techniques presented provide students with an excellent ‘resume builder’ and training for future career opportunities or graduate school in biotechnology. As a result each student prepares a resume based on their experience. The course dove-tails with a successful University/Industry interaction and an internship program allowing student’s additional summer support and training. This course may serve as a model for the development of future biotechnology courses in other areas.